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EcogeneTopic Page
TopicPage for Abasic site:G binding of Escherichia coli K-12
 | Proteome Chips: Mismatch Binding |
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Description:
Chen (2008) identified the top 20 proteins that preferentially bind to dsDNA containing an abasic site (N) unpaired to a G, called probe 4. Probe 1 is the same 19-mer with a G:C instead of the G-N.
Details:
5'-GGAATTAAAGNGAAGACC-3'
3'- CCTTAATTTCGCTTCTGG-5
The ratio of the preferential probe 4 binding ranges from 10.4 fold for YbaZ to 1.7 fold for YhaR.
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