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EcogeneTopic Page
TopicPage for Abasic site:A binding of Escherichia coli K-12
 | Proteome Chips: Mismatch Binding |
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Description:
Chen (2008) identified the top 20 proteins that preferentially bind to dsDNA containing an abasic site (N) unpaired to a A, called probe 5. Probe 1 is the same 19-mer with a G:C instead of the A-N.
Details:
5'-GGAATTAAAGNGAAGACC-3'
3'- CCTTAATTTCACTTCTGG-5
The ratio of the preferential probe 5 binding ranges from 13.2 fold for Dgt to 1.9 fold for YdcA. YbaZ is ranked second at 8.0 fold.
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