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EcogeneTopic Page
TopicPage for Abasic site:T binding of Escherichia coli K-12
 | Proteome Chips: Mismatch Binding |
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Description:
Chen (2008) identified the top 20 proteins that preferentially bind to dsDNA containing an abasic site (N) unpaired to a T, called probe 7. Probe 1 is the same 19-mer with a G:C instead of the T-N.
Details:
5'-GGAATTAAAGNGAAGACC-3'
3'- CCTTAATTTCTCTTCTGG-5
The ratio of the preferential probe 7 binding ranges from 11.8 fold for YbaZ to 1.8 fold for YcdC. Dgt is ranked second at 7.5 fold.
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